Chlorophyll analyzer need to pay attention to what issues?
In the field of modern agricultural research, the chlorophyll meter is a relatively common detection instrument. It has the advantages of convenient measurement, rapid measurement, and high precision. However, it should be noted that high precision has specific requirements, that is, it should be used only in accordance with the specifications. Can we get true and accurate data results, but for several factors affecting the determination of SPAD value by the chlorophyll analyzer, we have summarized several issues that need attention in the use of chlorophyll meter.
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1. In general, we often use the chlorophyll analyzer to determine the chlorophyll content of plants, and use this to determine the nitrogen content of plants, but in the process of measurement, it is critical to determine the critical value of SPAD, and under the same growth conditions. There is a big difference in SPAD values ​​among different varieties. Therefore, different SPAD thresholds must be established for different varieties. Thus, the nitrogen content measured by the chlorophyll meter may be accurate.
2. In the process of measuring with the chlorophyll meter, if the selected leaf position is different, the measurement results obtained may also be different. Therefore, in order to obtain a representative measurement result, first of all, the leaf position to be selected should be agreed. The few leaves on the top of the plant are relatively less affected by the nitrogen content in the soil, so the top leaves can be selected for use in chlorophyll meter measurements.
3. For the same blade, the SPAD values ​​measured at different positions are also different. For example, corn has the smallest deviation from the base in the middle of the blade at 55%, while it is measured in the 40% to 60% area. The coefficient of variation is lower. Therefore, in the process of measuring with the chlorophyll meter, it is also necessary to pay attention to the choice of the measurement orientation in order to avoid the measurement error.
In addition, during actual measurement using the chlorophyll meter, we also found that the intensity of light radiation has a certain influence on the measurement results of the chlorophyll meter, and as the illuminance of light radiation increases, the SPAD reading decreases. Therefore, during the measurement, the time for the determination should be reasonably selected so as to avoid the influence of the ecological environment on the determination of the chlorophyll meter.